diff --git a/test_runner/fixtures/neon_fixtures.py b/test_runner/fixtures/neon_fixtures.py
index 131820f23e..8f56ee4392 100644
--- a/test_runner/fixtures/neon_fixtures.py
+++ b/test_runner/fixtures/neon_fixtures.py
@@ -1274,6 +1274,8 @@ class NeonEnv:
 
             if self.pageserver_virtual_file_io_engine is not None:
                 ps_cfg["virtual_file_io_engine"] = self.pageserver_virtual_file_io_engine
+            if self.pageserver_virtual_file_io_mode is not None:
+                ps_cfg["virtual_file_io_mode"] = self.pageserver_virtual_file_io_mode
             if config.pageserver_default_tenant_config_compaction_algorithm is not None:
                 tenant_config = ps_cfg.setdefault("tenant_config", {})
                 tenant_config["compaction_algorithm"] = (